The obesity epidemic has resulted in an increased incidence of fatty liver diseases in our population, and this directly impacts organ donations. When transplanted, fatty livers place patients at a higher risk for graft dysfunction and lower survival rates. The gold standard to detect fat concentrations in donor livers is macroscopic assessment from a trained surgeon and with a liver biopsy. Unfortunately, a biopsy usually results in a significant delay in the organ retrieval process and subsequent transplantation. As such, many potentially viable donor livers are discarded based on a subjective assessment of fat content by surgeon’s visual inspection during organ retrieval; and conversely unviable ones may be transplanted. An accurate assessment of graft Hepatic steatosis (HS) during organ procurement is crucial in predicting liver graft post-transplant dysfunction risks. We developed a simple, hand-held Raman scattering analyzer for accurate real-time liver fat content assessment and evaluated it by scanning pork lard phantoms and fatty animal livers. High coefficients of determination of these evaluations highlight the capability of our device to accurately assess the level of steatosis of ex-situ and in-situ human livers.